| Autor: |
García, Trina H., da Rocha, Claudia Quintino, Dias, Marcelo J., Pino, Liudis L., del Barrio, Gloria, Roque, Annele, Pérez, Caridad E., dos Santos, Lourdes Campaner, Spengler, Iraida, Vilegas, Wagner |
| Zdroj: |
Natural Product Communications; January 2017, Vol. 12 Issue: 1 |
| Abstrakt: |
The flowers and leaves of Ageratina havanensis(Kunth) R. M. King & H. Robinson are traditionally used as a tea to cure several diseases. The production of active secondary metabolites can be affected by several environmental factors such as climate, altitude, rainfall, phenological stage and other conditions that may influence the growth of plants. In this sense, the development of a methodology to compare the chemical composition of plant extracts is needed. The qualitative chemical composition of the ethyl acetate extracts of flowers and leaves, collected in both reproductive and non-reproductive season, was determined by flow injection analysis-electrospray ionization-ion trap tandem mass spectrometry (FIA-ESI-IT-MSn) and ultra-high-performance liquid chromatography coupled to electrospray negative ionization mass spectrometry (UPLC/ESI-MSn). The qualitative chemical composition of the ethyl acetate extracts of flowers and leaves was very similar in all cases. Also the antiviral activity of flowers against human herpes simplex virusestype 1 and 2 (HSV-1, HSV-2) (Herpesviridae) was analyzed. Three glucoside flavonoids were isolated from the ethyl acetate extract of the leaves of A. havanensiscollected in flowering season using chromatographic methods and their structures were elucidated by physical and spectroscopic data measurements, and by comparing the obtained data with previously published values. The compounds were identified as 3-O-β-D-glucosyl-7-methoxyaromadendrin (5), 7-O-β-D-glucosyl-4′-dihydroxy-5-methoxyflavanone (6) and 5-O-β-D-glucosylsakuranetin (7); this is the first report of the isolation of these compounds in the Asteraceae family. Since the qualitative composition of the extracts of A. havanensiswas similar in all cases, it can be expected that the ethyl acetate extract of the leaves collected in the non-reproductive season has anti-herpetic activity similar to that obtained in the reproductive season. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
