| Abstrakt: |
The effects of NaCI 0.9, Ringeracetate, and Ringerdextran given as intravenous infusions in the microcirculatory changes observed in early stages of endotoxemia were investigated in male hamsters treated with Escherichia colilipopolysaccharide LPS. The cheek pouch was studied in vivoby means of intravital microscopy. Mean arterial MAP and venous pressures CVP, heart rate, mean arteriolar internal diameter, spontaneous arteriolar vasomotion AV, red blood cell velocity RBCV in these vessels, and longterm effects of LPS were evaluated in animals treated with either LPS alone or the combination of LPS with NaCI 0.9, Ringeracetate and Ringerdextran. The intravenous injection of LPS 0.3 mgkg elicited a significant reduction in MAP and CVP, cessation of AV and a decrease in RBCV. In our study, the heart rate and the arteriolar diameter did not change significantly, compared with the control values obtained before the LPS injection. No improvement in the MAP could be detected with infusions of NaCI 0.9 or Ringeracetate but the infusion of Ringerdextran increased it significantly. All infusions tested maintained the CVP until the end of the observation period and the Ringerdextran increased it significantly. The heart rate was maintained around 360 beatsmin with a tendency to decrease 70 min after the LPS infusion in all groups studied except the group which received NaCI 0.9 where the heart rate decreased significantly. In all the four groups, the mean arteriolar diameter did not change significantly with time during the observed period. RBCV decrease with the combination LPS NaCI 0.9 and the infusions of Ringeracetate and Ringerdextran maintained it until the end of the observation period. The combination of LPS NaCI 0.9 maintained the spontaneous arteriolar vasomotion during 50 min after LPS injection and the infusion of Ringeracetate maintained it for the 3h observation period. The infusion of Ringerdextran maintained the amplitude of the spontaneous arteriolar vasomotion and increased its frequency significantly. The longterm effects of LPS showed weight loss and pus on the periorbital area. Our results suggest that the best solution to maintain the microcirculatory parameters during the early stage of endotoxemia after LPS injection was the Ringerdextran. |
