| Autor: |
Armstead, Valerie E., Minchenko, Alexander G., Scalla, Rosario, Lefer, Allan M. |
| Zdroj: |
Shock; April 2001, Vol. 15 Issue: 4 p323-326, 4p |
| Abstrakt: |
Tissue factor TF is the primary cellular initiator of the coagulation protease cascade and serves as a cell surface receptor and a specific cofactor for plasma factors VIIVIIa. Because there is evidence that TF is regulated by a Pselectin dependent gene, we examined TF mRNA expression in the lungs during murine traumatic shock in the presence and absence of recombinant soluble Pselectin glycoprotein ligand1 rsPSGL.lgby using ribonuclease protection assays. Moreover, we studied the level of TF mRNA expression in mice with their Pselectin gene deleted Pselectin . Our data show that TF mRNA was significantly increased 143 P< 0.001 in the lungs 2 h after trauma compared with control rats subjected to sham trauma, which exhibited reduced TF mRNA expression −34 P< 0.001 after systemic administration of rsPSGL.lg. The expression of TF mRNA was also significantly decreased −29 P< 0.05 in the lungs of Pselectin mice compared with wildtype control C57B16 mice. The present results provide evidence for a Pselectindependent mechanism that enhances TF gene expression in traumatic shock. The major support for this mechanism is that either blockade of Pselectin by rsPSGL.Ig or deletion of the Pselectin gene leads to significant decreases in TF mRNA expression in the lung. These results are consistent with the concept that TF interacting with Pselectin may play a significant role in the pathophysiology of trauma. |
| Databáze: |
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