| Autor: |
Fazio, A., Artesi, C., Russo, M., Trio, R., Oteri, G., Pisani, F. |
| Zdroj: |
Therapeutic Drug Monitoring; December 1992, Vol. 14 Issue: 6 p509-512, 4p |
| Abstrakt: |
A sensitive, specific and rapid liquid-chromatographic method for the determination of the new antiepileptic drug lamotrigine (LTG) in human plasma is described. The method involves the use of a commercially available 3-μm particle size normal-phase column and a microflow-cell-equipped ultraviolet detector. Extraction is carried out with ethyl acetate after alkalinization on a 100-μl plasma sample containing LTG and 3,5-diamino-6-(2-methoxyphenyl)-1,2,4-triazine as internal standard. The residue is reconstituted with 50 μl of ethanol, and 5 μl of the final solution is injected into the column. Elution is carried out at 35°C using n-hexane/absolute ethanol/35 ammonia (80/20/0.25 by volume) as mobile phase at a flow rate of 2.0 ml/min. Detection is at 313 nm. The chromatographic separation requires 3 min and the sensitivity limit is 0.1 mg/L. Recovery is 88–96.2, whereas within-day and day-to-day coefficients of variation are between 4.1 and 7.7. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
