| Abstrakt: |
A simple, rapid, selective, and sensitive analytical method was developed for the quantification of atenolol in small volumes of plasma, by high-performance liquid chromatography with fluorescence detection. Only 200 μL of plasma was used for chromatographic analysis. Separation was performed on a C18 reverse-phase column (4 μm) using a binary mobile phase consisting of 0.05 M of phosphate buffer, pH 5.5, and methanol (8020, volvol) at a flow rate of 0.7 mLminute. The retention times of atenolol and of the internal standard (sotalol) were 12.7 and 10.4 minutes, respectively. Validation of this analytical method showed a good linear correlation (8–2000 ngmL), high sensitivity (quantification limit 8 ngml and detection limit 4 ngmL), accuracy of 99.3, and intraday and interday precision of 5.3 and 6.9, respectively. Absolute recovery was 93.7. The method was found to be robust, with acceptable stability. The analytical method was validated by the quantification of atenolol in plasma obtained from 2 patients with unstable angina, scheduled for myocardium revascularization surgery, who were chronically treated with 50 mg of atenolol administered per os once a day. The method developed was found to be adequate for use in pharmacokinetic studies and in adjusted dose pharmacotherapy. |
