Simultaneous Isocratic HPLC Determination of Vigabatrin and Gabapentin in Human Plasma by Dansyl Derivatization

Autor: Krivanek, Peter, Koppatz, Karl, Turnheim, Klaus
Zdroj: Therapeutic Drug Monitoring; June 2003, Vol. 25 Issue: 3 p374-377, 4p
Abstrakt: A rapid and low-cost assay for simultaneous vigabatrin (VGA) and gabapentin (GBP) determination is described that can be performed with simple HPLC instrumentation. The method involves derivatization of the primary amine group of VGA and GBP with dansyl chloride followed by isocratic separation (column Bondapak C-18, 10 m, 300 × 3.9 mm; mobile phase 50 mmol/L NaH2PO4in 40 acetonitrile) at 50°C and fluorometric detection (excitation and emission wavelength 318 and 510 nm, respectively) of the fluorescent product, which is stable for at least 7 days. Correlation coefficients of the calibration curves are >0.999 with a lower limit of detection of 0.3 g/mL. Between- and within-run coefficients of variation are below 4.5, and assay time is 15 minutes. This method may be used for therapeutic drug monitoring in the case of GBP and to control patient compliance in the case of VGA.
Databáze: Supplemental Index