A Paper Chromatoelectrophoresis Method for the Determination of Bilirubin Binding to Serum Protein

Autor: AINBENDER, EUGENE, DROWN, EDWIN G., LEE, YUN J., SWEET, AVRON Y.
Zdroj: Pediatric Research (Ovid); July 1977, Vol. 11 Issue: 7 p808-810, 3p
Abstrakt: A technique is described in which paper chromatoelectrophoresis is used to measure the bilirubin binding capacity of albumin in scrum. It is equivalent to the Sephadex G-25 method and its results are highly reproducible. From a single pool of serum, multiple determinations indicated the mean bilirubin binding capacity to be 23.7 ± 0.76 (SD) mg/dl and the greatest difference between any two values was 2 mg/dl. This new technique can determine the bilirubin binding capacity of a serum sample in 15 min. Highly skilled technicians are not needed and very small quantities of serum are required, which gives it a definite advantage over the Sephadex G-25 method.
Databáze: Supplemental Index