| Autor: |
Langham, Robyn G., Egan, Melissa K., Dowling, John P., Gilbert, Richard E., Thomson, Napier M. |
| Zdroj: |
Transplantation; December 2001, Vol. 72 Issue: 11 p1826-1829, 4p |
| Abstrakt: |
Cyclosporine nephropathy (CyAN) is a major limiting factor in the otherwise successful widespread use of cyclosporine in solid organ transplant. Transforming growth factor-1 (TGF-1) has been implicated as an important fibrogenic cytokine in the development of this disease. TGF--inducible gene-H3 (ig-H3) is a TGF-1- induced gene product, which acts as a marker for biologically active TGF-1. This study reports TGF-1 gene expression and ig-H3 tissue distribution in non-renal allograft CyAN. Renal tissue from nine patients who had developed CyAN after successful heart or heart-lung transplantation and from four kidneys removed for tumour were analyzed for TGF-1 gene expression ig-H3 protein with reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry, respectively. TGF-1 gene expression was increased in CyAN compared to nephrectomy (P<0.0001). ig-H3 protein expression was identified in distal convoluted tubular epithelium and parietal glomerular epithelium in CyAN, and not in nephrectomy samples. Expression of TGF-1 mRNA was significantly higher in renal tissue from patients not receiving angiotensin converting enzyme inhibitor (ACEI) therapy for hypertension (P<0.05). These findings support the hypothesis that TGF-1 is an important cytokine in the development of CyAN, independent of its role in chronic rejection in renal allografts. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
