| Autor: |
HAMMERBERG, CRAIG, WUDL, LINDA R., SHERMAN, MICHAEL I. |
| Zdroj: |
Transplantation; June 1980, Vol. 29 Issue: 6 p484-491, 8p |
| Abstrakt: |
T/T and tw18/tw18blastocysts appear to survive in culture for periods of 3 weeks at frequencies equal to that of normal embryos. We have, therefore, undertaken to generate cell lines with a homozygous mutant genotype. We attempted to identify these cells by H-2haplotype analysis. The results indicate that barring recombination, which would be expected to occur at a frequency of about 15, 1 of 10 blastocyst-derived cell lines from a (+/tw18× +/tw18) cross had a tw18/tw18genotype and 1 of 4 lines from a (T/+ × T/+) cross had a T/Tgenotype. Unexpectedly, all of the remaining lines were heterozygous; no +/+ lines were observed. The putative homozygous mutant lines might be useful in attempts to characterize tw18-specific antigens. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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