| Autor: |
Colley, Daniel G., Waksman, Byron H. |
| Zdroj: |
Transplantation; April 2023, Vol. 9 Issue: 4 p395-404, 10p |
| Abstrakt: |
Normal rabbit serum contains substances resembling antibodies which stain rat thymus cells by the indirect immunofluorescence technique and are cytotoxic for rat lymphoid cells in general. Separation of several normal rabbit sera on a Sephadex G-200 column yielded cytotoxic activity in either macroglobulin or both macroglobulin and globulin fractions. The cytotoxic properties of both fractions were found to be 2-mercaptoethanol-sensitive and heat-labile at 56 C for 30 min, and neither was dependent on added complement for cytotoxicity. Cytotoxicity was inhibited by horse antirabbit γ -globulin antiserum and in the presence of ethylene diamine tetraacetic acid. By the use of a quantitative absorption technique, the antigen responsible for cytotoxicity was shown to have a distinctive distribution in rat lymphoid tissues. There are proportionately larger quantities on thymus cells than on lymph node cells, peripheral white blood cells, spleen cells, or bone marrow cells, respectively. It is also present on kidney homogenates. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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