| Autor: |
Beukelman, C.J., Worm, E. van den, Dijk, H. van, Lafeber, F.P.J.G., Roy, J.L.A.M. van, Vianen, M.E., Roon, J.A.G. van, Bijlsma, J.W.J. |
| Zdroj: |
Rheumatology; November 1999, Vol. 38 Issue: 11 p1088-1093, 6p |
| Abstrakt: |
Objective. To investigate whether apocynin, 1-(4-hydroxy-3-methoxyphenyl)ethanone, is able to diminish inflammation-induced cartilage destruction in rheumatoid arthritis (RA), studied in a human in vitro model.Methods. Apocynin was added to cultures of RA peripheral blood mononuclear cells (PBMNC). Cartilage-destructive activity was determined by addition of culture supernatant to tissue samples of human articular cartilage. In addition, the proliferation of PBMNC, their production of tumour necrosis factor alpha (TN-Fα), interleukin (IL)-1 and IL-10, and T-cell production of interferon gamma (IFN-γ) and IL-4, as measures for T1 and T2 cell activity, were determined.Results. Apocynin was able to counteract RA PBMNC-induced inhibition of cartilage matrix proteoglycan synthesis, while no effect on inflammation-enhanced proteoglycan release was found. The effect was accompanied by a decrease in IL-1 and TNF-α production by the MNC. No effect on T-cell proliferation was found, but the production of IFN-γ, IL-4 and T-cell-derived IL-10 was strongly diminished. Most important, apocynin did not show any direct adverse effects on chondrocyte metabolism; on the contrary, it diminished the release of proteoglycans from the cartilage matrix.Conclusion. Apocynin in vitro inhibits inflammation-mediated cartilage destruction without having adverse effects on cartilage. The latter may be an advantage of apocynin over many other non-steroidal anti-inflammatory drugs. Therefore, apocynin might have an added beneficial effect in protecting RA patients from joint destruction. |
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