| Autor: |
Lussier, M., Véronneau, S., Sdicu, A-M., Bussey, H., Romero, P.A., Herscovics, A. |
| Zdroj: |
Glycobiology; October 1999, Vol. 9 Issue: 10 p1045-1051, 7p |
| Abstrakt: |
The genome of Saccharomyces cerevisiae contains five genes that encode type II transmembrane proteins with significant amino acid similarity to the α-1,3-mannosyltransferase Mnn1p. The roles of the three genes most closely related to MNN1 were examined in mutants carrying single and multiple combinations of the disrupted genes. Paper chromatographic analysis of [2-3H]mannose-labeled O-linked oligosaccharides released by β-elimination showed that the MNT2 (YGL257c) and MNT3 (YIL014w) genes in combination with MNN1 have overlapping roles in the addition of the fourth and fifth α-1,3-linked mannose residues to form Man4 and Man5 oligosaccharides whereas MNT4 (YNR059w) does not appear to be required for O-glycan synthesis. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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