| Abstrakt: |
WHOLE-CELL recordings were made from neurones in slices of rat olfactory cortex (10–19 days old), using potassium methylsulphate-filled pipettes. Positive commands applied from —60 mV in the presence of oxotremorine-M (10–20 μM, 20 of 30 cells) or trans-ACPD (10–50μM, 4 of 9 cells) evoked a slow inward tail current similar to the K+-mediated tail current (IK,ADP) recorded with sharp electrodes. IADPwas reduced by hyperpolarization, showed a 27 decrease in input conductance at its peak and was depressed by Cd2+, 4AP or high K+but unaffected by Cs+, Ba2+or TEA (5mM). IADPwas significantly larger in cells between postnatal days 13 and 15 than in younger neurones (10–12 days). These data show that stable whole-cell recordings of IADPcan be made from olfactory neurones in cortical slices, and support our hypothesis that IADPis a slowly reactivating, Ca2+-sensitive K+conductance. |
