| Autor: |
Olcese, J., Domagalski, R., Bednorz, A., Weaver, D. R., Urbanski, H. F., Reuss, S., Middendorff, R. |
| Zdroj: |
NeuroReport; March 2003, Vol. 14 Issue: 4 p613-618, 6p |
| Abstrakt: |
Hypothalamic GnRH (gonadotropin-releasing hormone) neurons play a critical role in the initiation and maintenance of reproduction competence. Using the mouse GnRH neuronal cell line, GT1-7, we have characterized the expression of the gene mPer1, a recognized key element of the mammalian circadian clockwork. Both mPer1transcripts and the 136 kDa mPER1 gene product could be detected in these cells. Immunocytochemical analysis also confirmed expression of mPER1 both in vitroand in vivoin GnRH neurons. Activation of cyclic AMP signalling pathways in vitroelevated GnRH secretion as well as mPer1expression and nuclear mPER1 immunoreactivity. As mPER1 is known to feedback on transcriptional activities in many cell models, the data presented here point to a role for mPER1 in the regulation of gene expression in GnRH neurons, and thus in the control of neuroendocrine activities. |
| Databáze: |
Supplemental Index |
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