| Autor: |
Jung, Bomi, Kim, Mi-Ok, Yun, Su-Jin, Lee, Eunjoo H. |
| Zdroj: |
NeuroReport; May 2003, Vol. 14 Issue: 6 p857-860, 4p |
| Abstrakt: |
Transforming growth factor (TGF)-1 is a key regulator of brain response to injury and inflammation. It exerts anti-inflammatory roles by inhibiting microglial proliferation and free radical induction. TGF-1 is known to induce apoptotic cell death of microglia in a Bcl-2-independent pathway. The purpose of this study was to examine detailed mechanisms of TGF-1-induced microglial apoptosis. Assays for cell viability and DNA fragmentation demonstrated that TGF-1 induced apoptotic cell death in primary rat microglial cultures. Reverse transcription (RT)-PCR analysis showed that primary microglial cells expressed mRNAs for rat inhibitor-of-apoptosis protein (RIAP)-1 and RIAP-3 under normal culture conditions and that treatment with TGF-1 resulted in a significant reduction in the amounts of RIAP-1 and RIAP-3 mRNAs. Because IAPs are potent suppressor of apoptotic cell death, decrease in IAP expression might provide an important regulatory function in TGF-1-mediated microglial death and in attenuation of excessive microglial activation in pathological conditions. |
| Databáze: |
Supplemental Index |
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