| Autor: |
Daadi, Marcel M., Pivirotto, Phillip, Bringas, John, Cunningham, Janet, Forsayeth, John, Eberling, Jamie, Bankiewicz, Krys S. |
| Zdroj: |
NeuroReport; February 2006, Vol. 17 Issue: 2 p201-204, 4p |
| Abstrakt: |
The present report describes for the first time, the stability of recombinant adeno-associated virus serotype 2 (AAV2) human aromatic L-amino acid decarboxylase (hAADC) gene transfer after 3-year survival time in a non-human primate model of Parkinson's disease. 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine-lesioned monkeys were treated with six injections of 30 μlsite of AAV2-hAADC at a concentration of 2×1012vgml into the caudate and putamen. Stereological analysis revealed a 46.6 increase in the total number of AAV2-hAADC-transduced cells in the striatum between 8 weeks and 3 years after gene transfer survival time. In the 8-week animals, the distribution of the AADC cells was dispersed and heterogeneous, whereas in the 3-year animals it was widespread and homogenous. Confocal analysis demonstrated that approximately 85 of the AADC cells were neuronal nuclei immunoreactive. |
| Databáze: |
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