| Abstrakt: |
Dipeptidyl peptidase IV (DPP-IV) converts glucagon-like peptide-1 (GLP-1)(7-36), responsible for glucose tolerance into inactive GLP-1(9-36). The pathogenic role of elevated adenosine deaminase (ADA) activity at hyperglycemic conditions was also suggested. Hence, the inhibition of both DPP-IV and ADA would be beneficial in the treatment of diabetes mellitus. Screening of the ability of water extracts of 23 Armenian Highland native plants to inhibit the enzymatic activity of DPP-IV and ADA was performed. Over 40% of DPP-IV and ADA activity inhibition was observed by 1 percent extracts from between 6 and 12 plants. Among them, the most effective inhibitor for DPP-IV inhibitor was the extract from the leaves of sea-buckthorn (Hippophaё rhamnoides) (80.5% ± 3.3; IC50 = 2.5mg/ml ± 0.03); and for ADA: from St. John's wort (Hypericum perforatum) (88.3% ±7.4; IC50 = 1.99mg/ml ± 0.3). Very slight ability of the known antidiabetic drugs, diabeton, glurenorm, siofor, glucovance, and the medicinal herbal mixture Arfazetin-A to inhibit the enzymes under study was observed. Moreover, the extracts from clove, cinnamon, green and black tea were highly effective in inhibiting DPP-IV and ADA. The obtained results show that selected Armenian Highland plants: blackberry, melilot; oregano, St. John's wort, sea-buckthorn leaves could be used, in combination with other antidiabetic drugs, for the treatment of diabetes mellitus. |
