| Autor: |
Swyka, Robert A., Berkowitz, David B. |
| Zdroj: |
Current Protocols in Chemical Biology; December 2017, Vol. 9 Issue: 4 p285-305, 21p |
| Abstrakt: |
The importance of discovering new chemical transformations and/or optimizing catalytic combinations has led to a flurry of activity in reaction screening. The in situenzymatic screening (ISES) approach described here utilizes biological tools (enzymes/cofactors) to advance chemistry. The protocol interfaces an organic reaction layer with an adjacent aqueous layer containing reporting enzymes that act upon the organic reaction product, giving rise to a spectroscopic signal. ISES allows the experimentalist to rapidly glean information on the relative rates of a set of parallel organic/organometallic reactions under investigation, without the need to quench the reactions or draw aliquots. In certain cases, the real‐time enzymatic readout also provides information on sense and magnitude of enantioselectivity and substrate specificity. This article contains protocols for single‐well (relative rate) and double‐well (relative rate/enantiomeric excess) ISES, in addition to a colorimetric ISES protocol and a miniaturized double‐well procedure. © 2017 by John Wiley & Sons, Inc. |
| Databáze: |
Supplemental Index |
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