| Autor: |
Andrade, Vicente, Flores, José, López, Miguel, Hernández, Andrés, Gómez, Sergio, Calvillo, Rosa, Martínez, Ana, Pérez, Juan, Hernández, Iván, Hidalgo, Erika, Osuna, Claudia, Jones, George, Guillén, Juan |
| Zdroj: |
Brazilian Journal of Microbiology; April 2019, Vol. 50 Issue: 2 p471-480, 10p |
| Abstrakt: |
American foulbrood (AFB) caused by Paenibacillus larvaeis the most destructive honeybee bacterial disease and its dissemination via commercial bee pollen is an important mechanism for the spread of this bacterium. Because Mexico imports bee pollen from several countries, we developed a tRNACys-PCR strategy and complemented that strategy with MALDI-TOF MS and amplicon-16S rRNA gene analysis to evaluate the presence of P. larvaein pollen samples. P. larvaewas not detected when the tRNACys-PCR approach was applied to spore-forming bacterial colonies obtained from three different locations and this result was validated by bacterial identification via MALDI-TOF MS. The genera identified in the latter analysis were Bacillus(fourteen species) and Paenibacillus(six) species.However, amplicon-16S rRNA gene analysis for taxonomic composition revealed a low presence of Paenibacillaceae with 0.3 to 16.2% of relative abundance in the commercial pollen samples analyzed. Within this family, P. larvaeaccounted for 0.01% of the bacterial species present in one sample. Our results indicate that the tRNACys-PCR, combined with other molecular tools, will be a useful approach for identifying P. larvaein pollen samples and will assist in controlling the spread of the pathogen. |
| Databáze: |
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