| Autor: |
Chen, Le, Yao, Shigang, Chen, Tao, Tao, Qin, Xie, Xiangting, Xiao, Xiang, Ding, Derong, He, Qin, He, Jian |
| Zdroj: |
Journal of Agricultural and Food Chemistry; January 2019, Vol. 67 Issue: 5 p1443-1452, 10p |
| Abstrakt: |
Dicamba, a broad-spectrum and highly efficient herbicide, is an excellent target herbicide for the engineering of herbicide-resistant crops. In this study, a new tetrahydrofolate (THF)-dependent dicamba methyltransferase gene, dmt50, was cloned from the dicamba-degrading strain Rhizorhabdus dicambivoransNdbn-20. Dmt50 catalyzed the methyl transfer from dicamba to THF, generating the herbicidally inactive product 3,6-dichlorosalicylic acid (3,6-DCSA) and 5-methyl-THF. A dmt50transgenic Arabidopsis thalianaclearly showed dicamba resistance (560 g/ha, the normal field application rate). However, Dmt50 demethylation activity was inhibited by the product 5-methyl-THF. Mthfr66, encoded by the 5,10-methylene-THF reductase gene mthfr66could relieve the inhibition by removing 5-methyl-THF in vitro. Compared with expression of dmt50alone, simultaneous expression of dmt50and mthfr66further improved the dicamba resistance (1120 g/ha) of transgenic A. thaliana. This study provides new genes for dicamba detoxification and a strategy for the engineering of dicamba-resistant crops. |
| Databáze: |
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