| Abstrakt: |
The following method has been devised for the production of experimental jejunal ulcer. The abdomen of a cat, under Nembutal anaesthesia, is opened and 3 adjacent segments, each 6-8 cm long, are cut from the first loop of the jejunum distal to the ligament of Treitz. A mesenteric pedicle of adequate size is chosen to ensure a good blood supply to each segment. L-shaped cannulae with rounded tips are tied into the ends of each segment. The sections of intestine are then replaced in the abdominal cavity, care being taken to avoid strangulation. The open ends of the cannulae are brought through stab incisions in the abdominal wall, which is then closed.The solutions to be perfused through the segments are pre-heated to 37-38°C. and are introduced via the distal cannulae to be drained proximally. A constant pressure of about 20 mm Hg is maintained by filling and inverting a 200-cc burette over a funnel leading to the heating jacket. The solutions used were (a) 0.1 N HC1, pH 1.2; (b) 3% pepsin (Parke, Davis & Co., 1:3,000) in 0.1 N HC1, pH 1.2; (c) 1 part colloidal aluminum hydroxide in 10 parts solution “b”, pH 3.5; (d) 1 part aluminum phosphate gel in 10 parts solution “b”, pH 2.0. The solutions are introduced at a rate of 1-2 cc per minute. After 12 hours, the sections of intestine are removed, examined grossly, and then fixed for histological study.The experimental data are shown in Table I. In all but one instance, blood appeared in the acid-pepsin perfusate during the first 2 hours. Perforation occurred 3 times as a result of perfusion with acid-pepsin. This solution always caused ulceration, which usually consisted of 2 large areas of ulceration at the points of contact of the intestinal mucosa and the tips of the cannulae. |
