| Autor: |
Quezada-Casasola, Andrés, Martínez-Armendáriz, Karla Eugenia, Itzá-Ortiz, Mateo Fabián, Escárcega-Ávila, Angélica María, Pérez-Eguía, Eduardo, Filipiak, Yael, Larocca, Clara, Carrera-Chávez, José María |
| Zdroj: |
Journal of Applied Animal Research; January 2018, Vol. 46 Issue: 1 p967-972, 6p |
| Abstrakt: |
ABSTRACTTrypan Blue (TB) and Brilliant Cresyl Blue (BCB) are used to select and determine viability and competence of oocytes, however, the effects of corpora luteaon cumulus expansion during in vitromaturation are still undetermined. Cumulus-oocytes complexes (COCs) from ovaries with ipsilateral (ICL), contralateral (CCL) and without corpora luteain either ovary (OCL) were selected by visual examination, TB and BCB staining, and matured in vitroto evaluate cumulus expansion. The overall percentage of visually selected COCs was similar in ICL, CCL and OCL (P > 0.05). Proportions of live and mature COCs were similar in all groups (P > 0.05). The overall percentage of BCB+ COCs was 70.1%. BCB+ ICL COCs had less cumulus expansion (60.5%) than BCB+ CCL and OCL COCs (75.7 and 71.4%, respectively; P < 0.01). BCB− ICL COCs had less cumulus expansion (20.0%) than BCB− CCL and OCL (39.7 and 46.1%; P < 0.01). BCB+ CCL and OCL COCs showed the highest cumulus expansion index (P < 0.01). Presence of corpus luteum in the ovary affects negatively cumulus cells expansion. TB and BCB staining facilitate the selection of oocytes with higher degrees of cumulus expansion. |
| Databáze: |
Supplemental Index |
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