| Autor: |
Augé-Gouillou, C., Hamelin, M.-H., Demattei, M.-V., Periquet, M., Bigot, Y. |
| Zdroj: |
Molecular Genetics and Genomics; March 2001, Vol. 265 Issue: 1 p51-57, 7p |
| Abstrakt: |
The two inverted terminal repeats (ITRs) flanking theMos-1 marinerelement differ in sequence at four positions. Gel retardation experiments indicated that each of these differences has a significant impact on the quality of the interaction between the ITR and theMos-1transposase. We showed that the transposase binds to the 3′ ITR better than to the 5′ ITR. The results of transposition assays performed inEscherichia coliindicated that these differences have an influence on the rate of transposition and the stability of the transposition products. Finally, we find that the wild-type configuration of theMos-1element, with one 5′ ITR and one 3′ ITR, is less efficient for transposition in bacteria than that of an element having two 3′ ITRs. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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