| Autor: |
Wiesak, T., Hunter, M. G., Foxcroft, G. R. |
| Zdroj: |
Reproduction; August 1992, Vol. 95 Issue: 3 p831-840, 10p |
| Abstrakt: |
Summary.Luteal cells were obtained by digestion of luteal tissue of cyclic (day 12) and early pregnant (days 12, 20 and 30) pigs. Suspensions of the dispersed luteal cells (5 × 104cells ml−1) were incubated for 2 h in minimum essential medium (MEM) alone (control) and MEM with different concentrations of prostaglandin F2α(PGF2α) and PGE2(0·01, 0·1, 1, 10, 100 and 1000 ng ml−1) and luteinizing hormone (LH) 100 and 1000 ng ml−1, or with combinations of LH + PGF2αand LH + PGE2. Net progesterone production was measured in the incubation media by direct radioimmunoassay.The overall response pattern of the luteal cells to exogenous hormones on day 12 of the oestrous cycle and pregnancy differed (P< 0·5) from treatment on day 20 and 30 of pregnancy. In general progesterone production was higher (P< 0·5) and the response to PGF2αand PGE2treatment was most obvious on day 12 of the oestrous cycle and pregnancy. Overall, PGF2αstimulated progesterone production in a dosedependent manner (P< 0·05). The response to PGE2was of a quadratic nature (P< 0·05) in which the lowest and the highest doses of PGE2were associated with a greater production of progesterone than were the intermediate doses. Treatment of luteal cells with PGF2α+ LH or PGE2+ LH caused overall inhibition (P< 0·05) of progesterone production compared with treatment with each hormone alone. This interaction was not affected by the dose of LH used.These findings indicate that PGF2αand PGE2are involved in the autocrine control of corpus luteum function.Keywords:pig; luteal cells; prostaglandins; pregnancy |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
