| Autor: |
Mújica, A., Moreno-Rodríguez, R., Naciff, J., Neri, L., Tash, J. S. |
| Zdroj: |
Reproduction; May 1991, Vol. 92 Issue: 1 p75-87, 13p |
| Abstrakt: |
Summary.Washed guinea-pig spermatozoa from the vas deferens re-acquired progressive motility within 1–2 min of incubation in minimal culture medium containing pyruvate and lactate. When glucose was added, either at the beginning or after 15 min of incubation, the cells showed stimulated motility (increased straight-line velocity, linearity and beat-cross frequency, P< 0·01). Re-acquisition of progressive motility was preceded by a significant (P< 0·005) transient increase in sperm concentration of cyclic adenosine 5′-phosphate (cAMP) with or without glucose in the medium. Papaverine caused another large significant (P< 0·001) increase in cAMP concentration; and 5·56mm glucose with papaverine caused a further stimulation in cAMP beyond that with papaverine alone (P< 0·005). Although 0·05 or 5·56 mmglucose plus α-chlorohydrin stimulated sperm motility, they did not further stimulate the increase in cAMP after 30 s of incubation. Thus, there was no apparent correlation between the glucose-stimulating effect on sperm motility and the enhancement of cAMP at 30 s. However, there was a close correlation between glucose-stimulated motility and enhancement of ATP (P< 0·05) by glucose even under incubation conditions in which glucose caused the Crabtree effect (decrease in respiration rate).Keywords:spermatozoa; motility regulation; glucose; ATP; cAMP; α-chlorohydrin; guinea-pig |
| Databáze: |
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