| Abstrakt: |
Summary.This study was conducted to test the hypothesis that the rate (dose/time) at which oestradiol-17β (oestradiol) is presented to the hypothalamo–pituitary axis influences secretion of LH, FSH and prolactin. A computer-controlled infusion system was used to produce linearly increasing serum concentrations of oestradiol in ovariectomized ewes over a period of 60 h. Serum samples were collected from ewes every 2 h from 8 h before to 92 h after start of infusion, and assayed for oestradiol, LH, FSH and prolactin. Rates of oestradiol increase were categorized into high (0·61–1·78 pg/h), medium (0·13–0·60 pg/h) and low (0·01–0·12 pg/h). Ewes receiving high rates of oestradiol (N = 11) responded with a surge of LH 12·7 ± 2·0 h after oestradiol began to increase, whereas ewes receiving medium (N = 15) and low (N = 11) rates of oestradiol responded with a surge of LH at 19·4 ± 1·7 and 30·9 ± 2·0 h, respectively. None of the surges of LH was accompanied by a surge of FSH. Serum concentrations of FSH decreased and prolactin increased in ewes receiving high and medium rates of oestradiol, when compared to saline-infused ewes (N= 8; P< 0·05). We conclude that rate of increase in serum concentrations of oestradiol controls the time of the surge of LH and secretion of prolactin and FSH in ovariectomized ewes. We also suggest that the mechanism by which oestradiol induces a surge of LH may be different from the mechanism by which oestradiol induces a surge of FSH.Keywords:oestradiol; LH; FSH; prolactin; ovariectomized ewes |
