| Autor: |
Osinowo, O. A., Bale, J. O., Oyedipe, E. O., Eduvie, L. O. |
| Zdroj: |
Reproduction; July 1982, Vol. 65 Issue: 2 p389-394, 6p |
| Abstrakt: |
Summary.A concentration of 0·005% formaldehyde in phosphate-buffered saline (PBS) achieved complete immobilization of ram spermatozoa while also yielding good recovery of sperm motility after removal by washing. At a higher formaldehyde concentration (0·01%) recovery rate declined with increasing dilution rate.Incubation of spermatozoa in PBS containing 0·005% formaldehyde beyond 6 h at 5, 15 or 25°C resulted in poor recovery rates. Of the incubation temperatures, eosin uptake was lowest at 25°C. During 4 h post-wash incubation at 30°C sperm motility was significantly (P< 0·001) affected by pre-wash formaldehyde concentration which had no effect on the proportion of eosinophilic spermatozoa. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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