| Autor: |
TORBIT, C. A., WEITLAUF, H. M. |
| Zdroj: |
Reproduction; January 1975, Vol. 42 Issue: 1 p45-50, 6p |
| Abstrakt: |
`Normal' and `delayed' implanting' mouse blastocysts were incubated in non-radioactive culture medium for various lengths of time (preincubation), and then placed in culture medium with [14C]-glucose for 2 hr. The rate of embryonic CO2production was estimated from 14CO2given off by the blastocysts. Normal embryos were unaffected by the length of preincubation. By contrast, `delayed implanting' embryos had a low level of CO2production with short preincubations (i.e. less than 2 hr), but approached the normal range with longer preincubations (i.e. more than 8 hr) and thus, were `activated' in vitro. Incubation of the `delayed implanting' embryos with actinomycin D prevented the expected increase in CO2production, indicating that synthesis of new RNA is necessary for their activation. Preincubation of normal and `delayed implanting' blastocysts with oestradiol-17β and/or progesterone had no effect on the level of CO2production, suggesting that the hormones do not directly stimulate or inhibit carbohydrate metabolism in the embryos. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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