| Abstrakt: |
1. The reducing method of Talbot, Saltzman, Wixom & Wolfe [1945], and the formaldehyde method of Corcoran & Page [1948], as well as a modification of that of Daughaday, Jaffe & Williams [1948] for the estimation of urinary corticosteroids, have been critically examined.2. A study has been made of the distribution of reducing material, of formaldehydogenic material and of reducing and Δ4-3-ketosteroids, in the various fractions obtained during the various purification procedures applied to the crude CHCl3extract.3. Caustic soda washing does not remove significant quantities of the steroids investigated, although non-steroid reducing and uncharacterized formaldehydogenic material is removed.4. The water fraction of the benzene-water partition contains all the reducing and Δ4-3-ketosteroids so far detected in urine.5. The formaldehydogenic method of Corcoran & Page gives slightly higher results than the modified method of Daughaday et al.6. The acid hydrolysis curves obtained with the Talbot method resembled those described by Paterson, Cox & Marrian [1950], but no regular form of hydrolysis curve was obtained when either formaldehydogenic method was used.7. A comparison of normal values obtained by the authors using the chemical and paper chromatography techniques revealed that the formaldehydogenic methods estimate a very high proportion of uncharacterized material, whereas results by the reducing method of Talbot et al. approximate relatively closely to those obtained by semiquantitative paper chromatography. |
