| Autor: |
DE COURCY, CONSTANCE |
| Zdroj: |
Journal of Endocrinology; October 1956, Vol. 14 Issue: 2 p164-NP |
| Abstrakt: |
1. Pregnane-3α:17α:20-triols give a blue fluorescent spot after heating a chromatogram sprayed with approximately 25% trichloracetic acid in ethanol. A spot containing 2 μg may be detected by this reaction. Out of forty-eight steroids examined only atiocholanediol gave a similar fluorescence. Certain other steroids give different characteristic colours and fluorescence.2. The test has been successfully used to detect pregnanetriol in the non-ketonic fraction of urine extracts from a normal male and from a patient with adrenal hyperplasia. It has been used to detect the production of pregnanetriol by in vitroreduction of pregnane-3α:17α-diol-20-one.3. The identity of the substances which gave this reaction and which were obtained from urine and from in vitroreduction was confirmed by isolation and characterization. |
| Databáze: |
Supplemental Index |
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