| Abstrakt: |
Radioimmunological methods for the assay of trophic hormones require antigens of high purity and specific activity. In a recent report Wilde & Bagshawe (1965) described an HCG preparation derived from the urine of patients with trophoblastic tumours which, after chromatography on DEAE-cellulose and Sephadex G-100, had a specific activity of approximately 23,000 i.u. (13,100–48,000) by bioassay; immunoassay results were considerably lower.In an attempt to obtain an HCG preparation of similar activity we have also used urine from a patient with trophoblastic disease. The extremely high initial titre permitted the use of simplified procedures for purification.Immunological assays were performed by serial dilution of a latex agglutination slide test (Goss & Taymor, 1965), and bioassays were performed by the ventral prostate method (Loraine, 1950). Protein concentration was estimated by a method previously outlined (Blatt, Park, Taymor & Todd, 1966).The immunological estimates of the activities of the original material and |
