| Autor: |
TOWNSEND, J., DUFFUS, W.P.H., WILLIAMS, D.L. |
| Zdroj: |
Research in Veterinary Science; September 1988, Vol. 45 Issue: 2 p198-205, 8p |
| Abstrakt: |
Peripheral blood mononuclear cells (pbmc) from calves infected with bovine herpesvirus type 1 (bhv1) or parainfluenza 3 virus (pi3) were cultured in vitro in the presence of inactivated specific antigen presented on mdbkcells. In the presence of inactivated antigen, PBMC from both bhv1-infected and control calves produced interferon (ifn)-α in 24 hour cultures. Altering the culture conditions did not result in the detection of immune-specific ifnproduced by mononuclear cells from bhv1-infected calves. However, spontaneous ifnwas detected in the absence of antigen in 24 hour cultures from infected animals: this ifnwas pH 2 labile and completely neutralised by antiserum to recombinant bovine ifn-γ. Spontaneous ifn-γ production was only seen in calves following a second bhv1 inoculation, given four to seven weeks after the primary dose. In contrast pbmccultures from PI3 virus-infected calves did not produce ifn-γ spontaneously, but did so in cultures which contained inactivated pi3 antigen. Mononuclear cells from control animals failed to produce either ifn-α or -γ when cultured with inactivated pi3 virus. ifn-γ was detected in pbmccultures after the primary infection, with no increase in production occurring following subsequent pi3 virus inoculations. Immunospecific production of ifn-γ provides a simple method for monitoring cell-mediated immunity in bhv1-and pi3 virus-infected calves and can be used for evaluating the efficacy of vaccines against these viruses. |
| Databáze: |
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