INTERFERENCE BY SERUM PROTEINS WITH LH RADIOIMMUNOASSAY USING IMMUNOSORBENT

Autor: Reuter, A. M., Hendrick, J. C., Sulon, J., Franchimont, P.
Zdroj: European Journal of Endocrinology; February 1973, Vol. 72 Issue: 2 p235-242, 8p
Abstrakt: The percentage of LH* bound to antibodies that have been covalently bound to cellulose is diminished in the presence of LH-free human serum and sera from various species of animals.Serum fractionation studies on Sephadex G 200 show that the greatest interference comes from the proteins eluted in the void volume i. e. the high molecular weight proteins. Specifically, the gamma M globulins and the α2-macroglobulins appear to play an important role, as demonstrated by tests in which these proteins were neutralized by gamma M and α2-macroglobulin antisera.
Databáze: Supplemental Index