| Autor: |
Tsukahara, Fujiko, Nomoto, Teruko, Maeda, Michiko |
| Zdroj: |
European Journal of Endocrinology; January 1989, Vol. 120 Issue: 1 p69-74, 6p |
| Abstrakt: |
Abstract. To characterize rT35′-deiodinase (5′D) in rat skeletal muscle, the effects of altered thyroid status and PTU on rT35′D were studied. rT35′D activity was measured by incubating homogenates of rat skeletal muscle with [125]rT3, iodine labelled in the outer ring, in the presence of 20 mmol/l DL-dithiothreitol. This activity was observed to increase significantly 24 h after a single scinjection of T3(75 μg/kg). The increase following the daily administration of this drug (15 or 75 μg/kg) for 3 and 14 days was dependent on the dose and number of previous days of injection. A significant decrease in activity was observed 2 weeks after thyroidectomy. The addition of 0.1 mmol/l 6-n-propyl-2-thiouracil (PTU) to the incubation medium in vitro caused a marked reduction in the activity in homogenates of skeletal muscle from hypothyroid, euthyroid and hyperthyroid rats. PTU, present at 0.05% in the drinking water for 2 weeks virtually abolished it. The properties of rT35′D in rat skeletal muscle thus appear to be essentially the same as those of type I enzyme with respect to response toward altered thyroid status and PTU. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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