| Autor: |
Trinh, Kieu The Loan, Minh Chon, Nguyen, Lee, Nae Yoon |
| Zdroj: |
Analytical Methods; 2018, Vol. 10 Issue: 42 p5071-5077, 7p |
| Abstrakt: |
In this study, we fabricated an integrated polystyrene (PS) microdevice for adhering Escherichia coliO157:H7 by exploiting the pristine PS surface as a pre-concentration step and subsequent amplification using a continuous flow polymerase chain reaction (CF-PCR) for rapid on-site detection. The PS surface, which contained styrene groups, could adhere to bacteria viaelectrostatic and van der Waals interactions. Subsequently, integration of pre-concentration and amplification using CF-PCR on the PS microdevice was demonstrated for rapid bacterial detection. The introduced method can be an easy-to-use platform for the rapid and simple detection of foodborne pathogens in real samples. |
| Databáze: |
Supplemental Index |
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