| Autor: |
Fujita, Chiyo, Kasai, Chihiro, Kosuge, Hiromi, Ogata, Kenji, Oshima, Ichiyo, Shima, Kenji |
| Zdroj: |
Annals of Clinical Biochemistry; September 1989, Vol. 26 Issue: 5 p416-421, 6p |
| Abstrakt: |
A monoclonal antibody (E-21) was obtained that specifically binds to human pancreatic amylase and shows negligible cross-reaction with human salivary amylase. Using this antibody a radioimmunoassay was developed for pancreatic amylase in human serum. The assay was shown to be sensitive (detectable up to 7 mg/L), reproducible, and specific for pancreatic amylase. In normal subjects, the mean concentration of serum pancreatic amylase determined by this method was 36·3 mg/L with a 95% confidence range of 16·5 to 79·2 mg/L. A good correlation was observed between the concentrations of immunoreactive pancreatic amylase (IR-PA) and enzymatic activities in 20 serum samples (r= 0·97). The concentration of serum IR-PA was below the detectable limit in pancreatectomised patients, and was greatly increased in patients with acute pancreatitis; the latter was accompanied by parallel changes in total enzymic activity. In patients with mumps, the serum IR-PA level was within the normal range whereas the total enzymic activity was elevated. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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