| Autor: |
Chen, Linda H., Packett, Leonard V. |
| Zdroj: |
The American Journal of Clinical Nutrition; October 1971, Vol. 24 Issue: 10 p1232-1237, 6p |
| Abstrakt: |
The in vitro effect of peroxidized linoleic acid on β-glucuronidase was studied. Rat liver cell preparation with the nucleus and cell membrane removed served as a source of β-glucuronidase. It was incubated at 25 C with peroxidized linoleic acid or its potassium salt, then β-glucuronidase activity was determined. Peroxidized linoleic acid or its potassium salt lowered this enzyme activity, but this reduction in activity was partially reversed with time of incubation. The inhibition of β-glucuronidase was prevented by α-tocopherol or glutathione. Two synthetic antioxidants (butyl hydroxytoluene, butyl hydroxyanisole) had less effect in preventing it. Ascorbic acid had no effect. Cysteine inhibited β-glucuronidase. Tocopherol-deficient rat liver preparation tended to have lower β-glucuronidase activity (P< 0.10) and higher thiobarbituric acid values (P< 0.01) than that of the control group. The same amount of peroxide inhibited β-glucuronidase of the tocopherol-deficient rat liver preparation more than that of control (P< 0.01). Addition of α-tocopherol in vitro to tocopherol deficient rat liver preparation prevented this further inhibition of β-glucuronidase caused by α-tocopherol deficiency. The results suggested that α-tocopherol reverses the inhibition of β-glucuronidase activity by reacting with the peroxide or an enzyme-peroxide complex. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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