| Autor: |
e Silva, Kleber SF, da S Neto, Benedito R, Zambuzzi-Carvalho, Patrícia F, de Oliveira, Cecília MA, Pires, Ludmila B, Kato, Lucilia, Bailão, Alexandre M, Parente-Rocha, Juliana A, Hernández, Orville, Ochoa, Juan GM, de A Soares, Célia M, Pereira, Maristela |
| Zdroj: |
Future Microbiology; October 2018, Vol. 13 Issue: 13 p1473-1496, 24p |
| Abstrakt: |
Aim:To perform the proteomic profile of Paracoccidioides lutziiafter treatment with the compound camphene thiosemicarbazide (TSC-C) in order to study its mode of action. Methods:Proteomic analysis was carried out after cells were incubated with TSC-C in a subinhibitory concentration. Validation of the proteomic results comprised the azocasein assay, western blot and determination of the susceptibility of a mutant to the compound. Results:Proteins related to metabolism, energy and protein fate were regulated after treatment. In addition, TSC-C reduces the proteolytic activity of the protein extract similarly to different types of protease inhibitors. Conclusion:TSC-C showed encouraging antifungal activity, working as a protease inhibitor and downregulating important pathways impairing the ability of the fungi cells to produce important precursors. |
| Databáze: |
Supplemental Index |
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