| Autor: |
Falke, Lee C., Gillis, Kevin D., Pressel, David M., Misler, Stanley |
| Zdroj: |
FEBS Letters; July 1989, Vol. 251 Issue: 1-2 p167-172, 6p |
| Abstrakt: |
We describe the application of ‘perforated patch recording’ using the pore‐forming antibiotic nystatin, to monitor the electrical activity and underlying ionic currents of rat and human pancreatic islet B cells. We demonstrate that glucose‐induced electrical activity is seen even in single B cells during current‐clamp recordings lasting hours ‘L‐type’ Ca2+‐channel currents can also be monitored over this period of time. This technique may prove useful in examining hormone and neurotransmitter modulation of electrical activity in B cells, while minimizing the effects of cytoplasmic ‘wash‐out’. |
| Databáze: |
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