| Autor: |
Convents, André, De Backer, Jean-Paul, Van Driessche, Edilbert, Convents, Daniel, Beeckmans, Sonia, Vauquelin, Georges |
| Zdroj: |
FEBS Letters; July 1988, Vol. 234 Issue: 2 p480-484, 5p |
| Abstrakt: |
α2,‐Adrenergic receptors in calf retina membranes can be specifically labeled with the tritiated agonist p‐azido[3H]clonidine. Saturation binding in the dark occurs with high affinity (1.3 ± 0.3 nM) to a single class of sites (1122 ± 67 fmol/mg protein). Irradiation of the membrane‐bound radioligand results in the labeling of a peptide band with an apparent size of 65 kDa and a characteristic pharmacological profile for an α2‐adrenergic receptor. The carbohydrate moieties of the α2‐receptor are characterized by lectin affinity chromatography and glycosidase treatment. The Nonidet P‐40‐solubilized, p‐azido[3H]clonidine‐labeled receptors are completely retained by Con A‐ as well as WGA‐Sepharose columns. Neuraminidase, α‐mannosidase and TFMS do not affect the electrophoretic mobility of the receptor on SDS‐PAGE whereas endoglycosidase F reduces the apparent size to 45 kDa. |
| Databáze: |
Supplemental Index |
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