| Autor: |
Boustead, Catherine M., Smallwood, Margaret, Small, Hazel, Bowles, Dianna J., Walker, John H. |
| Zdroj: |
FEBS Letters; February 1989, Vol. 244 Issue: 2 p456-460, 5p |
| Abstrakt: |
Calcium‐dependent phospholipid‐binding proteins of apparent Mr33 000 and 35 000 were isolated from suspension cultures of tomato cells. Two‐dimensional gel electrophoresis showed the proteins to have isoelectric points of approx. 5.7 and 5.6, respectively. In the presence of calcium, both proteins bound to liposomes formed from a mixture of phosphatidylserine and phosphatidylcholine, but not to liposomes of phosphatidylcholine alone. Both proteins showed immunological similarities to previously characterized calcium‐dependent phospholipid‐binding proteins (annexins) from Torpedo marmorataand mammalian species. The protein of Mr33 000 cross‐reacted with three separate antisera raised to the annexin Torpedo calelectrin, whereas that of Mr35 000 cross‐reacted with antisera to the bovine annexins p68 and p32/34. We suggest that the two proteins may represent the first identification in higher plants of the annexin family of calcium‐dependent phospholipid‐binding proteins. |
| Databáze: |
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