| Autor: |
Fukumori, Fumiyasu, Takeuchi, Naomi, Hagiwara, Toshihiko, Ito, Katumi, Kochibe, Naohisa, Kobata, Akira, Nagata, Yoshiho |
| Zdroj: |
FEBS Letters; July 1989, Vol. 250 Issue: 2 p153-156, 4p |
| Abstrakt: |
Aleuria aurantialectin (AAL) shows sugar‐binding specificity for L‐fucose. A λgt11 expression library was constructed from A. aurantiapoly(A) RNA and screened with a polyclonal antiserum directed against AAL. An immunopositive clone carrying 1.3‐kb EcoRI fragment was obtained. The fragment encoded AAL, but lacked a nucleotide sequence corresponding to the two amino‐terminal amino acids. The 5′‐terminal part of the fragment was replaced with a chemically synthesized DNA fragment and inserted into an expression vector to yield a plasmid pKA‐1. Escherichia colicarrying pKA‐1 expressed functional AAL and the recombinant AAL showed the same immunological properties as those of natural AAL. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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