| Autor: |
Rosario Fernández, M., Biosca, Josep A., Norin, Annika, Jörnvall, Hans, Parés, Xavier |
| Zdroj: |
FEBS Letters; August 1995, Vol. 370 Issue: 1-2 p23-26, 4p |
| Abstrakt: |
Alcohol dehydrogenase class III (glutathione‐dependent formaldehyde dehydrogenase) from Saccharomyces cerevisiaewas purified and analyzed structurally and enzymatically. The corresponding gene was also analyzed after cloning from a yeast genome library by screening with a probe prepared through PCR amplification. As with class III alcohol dehydrogenase from other sources, the yeast protein was obtained in two active forms, deduced to reflect different adducts/modifications. Protein analysis established N‐terminal and C‐terminal positions, showing different and specific patterns in protein start positions between the human/mammalian, yeast, and prokaryotic forms. Kmvalues with formaldehyde differ consistently, being about 10‐fold higher in the yeast than the human/mammalian enzymes, but compensated for by similar changes in κcatvalues. This is compatible with the different functional needs, emphasizing low formaldehyde concentration in the animal cells but efficient formaldehyde elimination in the microorganisms. This supports a general role of the enzyme in formaldehyde detoxication rather than in long‐chain alcohol turnover. |
| Databáze: |
Supplemental Index |
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