| Abstrakt: |
We recently identified 1α,25‐dihydroxy‐3‐epi‐vitamin D3as a major in vitro metabolite of 1α,25‐dihydroxyvitamin D3, produced in primary cultures of neonatal human keratinocytes. We now report the isolation of 1α,25‐dihydroxy‐3‐epi‐vitamin D3from the serum of rats treated with pharmacological doses of 1α,25‐dihydroxyvitamin D3. 1α,25‐dihydroxy‐3‐epi‐vitamin D3was identified through its co‐migration with synthetic 1α,25‐dihydroxy‐3‐epi‐vitamin D3on both straight and reverse phase high performance liquid chromatography systems and by mass spectrometry. Along with 1α,25‐dihydroxy‐3‐epi‐vitamin D3, other previously known metabolites, namely, 1α,24(R),25‐trihydroxyvitamin D3, 1α,25‐dihydroxy‐24‐oxo‐vitamin D3and 1α,25‐dihydroxyvitamin D3‐26,23‐lactone, were also identified. Thus, our study for the first time provides direct evidence to indicate that 1α,25‐dihydroxy‐3‐epi‐vitamin D3is an in vivo metabolite of 1α,25‐dihydroxyvitamin D3in rats. |
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