Promoter and expression studies on an Arabidopsis thalianadehydrin gene

Autor: Rouse, Dean T., Marotta, Rosetta, Parish, Roger W.
Zdroj: FEBS Letters; March 1996, Vol. 381 Issue: 3 p252-256, 5p
Abstrakt: A genomic clone of a group 2 lealrabldehydringene from Arabidopsis thaliana, Xero2llti30, was cloned and sequenced. Promoter‐GUSfusions were introduced into plants to analyse the promoter and determine expression patterns. Using root cultures, GUSexpression was found to be moderately stimulated by abscisic acid (ABA), wounding, cold and dehydration. Results with an ABA‐deficient mutant suggested endogenous ABA is required for these responses. Promoter deletion studies indicated multiple cis‐acting elements are involved in the induction of the gene. GUSexpression occurred in desiccated seeds, in all tissues of young seedlings and in roots (with the exception of the root tip), desiccated pollen grains, trichomes and the vascular tissues of leaves and stems in mature plants.
Databáze: Supplemental Index