| Autor: |
Gershburg, Eduard, Stockholm, Daniel, Froy, Oren, Rashi, Sharon, Gurevitz, Michael, Chejanovsky, Nor |
| Zdroj: |
FEBS Letters; January 1998, Vol. 422 Issue: 2 p132-136, 5p |
| Abstrakt: |
The insecticidal efficacy towards Helicoverpa armigeralepidopteran larvae of recombinant Autographa californicaM nucleopolyhedroviruses, expressing depressant and excitatory scorpion anti‐insect selective toxins, was investigated. The ET50(effective paralysis time 50%) values obtained with the recombinant viruses expressing the depressant toxin, LqhIT2, and the excitatory toxin, LqhIT1, were 59 h and 66 h, respectively, whereas the ET50value of the wild‐type virus was longer, 87 h post infection. The insecticidal effects obtained when using two distinct temporally regulated viral promoters revealed advantage for the very late p10 promoter over the p35 early promoter. The higher insecticidity of the virus expressing the depressant toxin compared to the excitatory toxin suggests that pharmacokinetic factors and/or promoter efficiency may play a role during infection of insect pest larvae by recombinant baculoviruses. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
Plný text