Human liver RNA‐programmed in vitro synthesis of a polypeptide related to human apolipoprotein B

Autor: Olofsson, Sven-Olof, Elias, Per, Boström, Kristina, Lundholm, Kent, Norfeldt, Per-Ingvar, Wiklund, Olov, Fager, Gunnar, Bondjers, Göran
Zdroj: FEBS Letters; May 1983, Vol. 156 Issue: 1 p63-66, 4p
Abstrakt: In an in vitro synthesizing system programmed with RNA from human liver a polypeptide with an estimated Mrof 80000 (80 kDa)±1400 (mean±SD, n=5) was synthesized. This polypeptide could be precipitated with antiserum to a narrow density cut of LDL (d=1.030‐1.055) or antiserum against the high‐Mrform of apoB (apoB 100 [4]). The synthesized protein is immunologically related to a 75 kDa protein isolated from LDL. We suggest that the 80 kDa protein represents a primary translation product of apoB synthesized in human liver.
Databáze: Supplemental Index