| Autor: |
Nitta, Noriko, Kuge, Osamu, Yui, Seiko, Tsugawa, Akiko, Negishi, Kazuo, Hayatsu, Hikoya |
| Zdroj: |
FEBS Letters; January 1984, Vol. 166 Issue: 1 p194-198, 5p |
| Abstrakt: |
Cytosine in nucleic acids can be converted into N4‐aminocytosine by treatment with a mixture of hydrazine and bisulfite. The hydrazino group thus formed at position 4 of the pyrimidine ring can be linked to a sulhydryl group in proteins by the use of bromopyruvate as a linker. Successful use of this scheme of chemical cross‐linking between nucleic acid and protein was demonstrated in the linking of poly(C) with glutathione, and of RNA with protein in the E. coli30 S ribosomal subunit. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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