| Abstrakt: |
While extralenticular expression of proteins in the α crystallin (small heat shock protein) family is well documented, that for proteins in the β/γ‐superfamily is less well established. Here we show, using SDS‐PAGE, Western blotting and confocal microscopy, that there is a constitutive level of β crystallin expression in mouse N1E‐115 neural cells. Furthermore, upon heat shock at 43°C or 55°C, or cold shock at 30°C, β crystallin immunoreactivity translocated predominantly from the nuclear region into the cytoplasmic region of the cells. In conditions of stress, it may be important for β crystallin to be recruited into the cytoplasm to stabilise other proteins via its high β‐sheet content, and/or to ensure that storage levels of cytoplasmic Ca2+are maintained. |
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