| Autor: |
Kumagai, Takanori, Nakano, Tetsuji, Maruyama, Masafumi, Mochizuki, Hiroshi, Sugiyama, Masanori |
| Zdroj: |
FEBS Letters; January 1999, Vol. 442 Issue: 1 p34-38, 5p |
| Abstrakt: |
The transposon Tn5carries a gene, ble, which confers resistance to bleomycin (Bm) and gives a survival advantage to its host cell. We found that the blegene product, designated BLMT, is a binding protein with a strong affinity for Bm. BLMT quenched both the antibacterial and DNA‐cleaving activities of Bm, when incubated with the antibiotic. An electron spin resonance spin‐trapping analysis showed that BLMT inhibits the generation of Bm‐induced hydroxyl radical, by trapping Bm but not the hydroxyl radical. Western blot analysis using an anti‐BLMT monoclonal antibody revealed that BLMT is immunologically distinct from Bm‐binding proteins from Streptomyces verticillus, Staphylococcus aureusand Streptoalloteichus hindustanus. Escherichia coli, transformed with a mutant blehaving leucine instead of proline at N‐terminal amino acid position 7, lost resistance to Bm, although the cell maintained the survival benefit. This suggests that the Bm resistance mediated by bleis independent of its ability to give a survival advantage to the host bacterium. |
| Databáze: |
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